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beas 2b cell  (ATCC)


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    ATCC beas 2b cell
    Beas 2b Cell, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4012 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/crl+3588/BEAS-2B/pm42320433-336-0-7
    Average 99 stars, based on 4012 article reviews
    beas 2b cell - by Bioz Stars, 2026-09
    99/100 stars

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    Article Title: Multi-omics profiling reveals microenvironmental remodeling as a key driver of house dust mite-induced lung cancer progression
    Article Snippet: Human: BEAS-2B cells , ATCC , Cat# CRL-3588, RRID:CVCL_0168.



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    Experimental validation of HLA-DPA1 and HLA-DPB1 downregulation. (A) BEAS-2B human bronchial epithelial cells were treated with IL-13 (20 ng/mL) for 24 h to establish an in vitro asthma cell model. Quantitative real-time PCR analysis showed that the mRNA expression levels of HLA-DPA1, HLA-DPB1 and CIITA were significantly decreased in the IL-13-treated group compared with the control group. (B) BEAS-2B human bronchial epithelial cells were treated with IL-13 (20 ng/mL) for 24 h to establish an in vitro asthma cell model. Western blot analysis showed that the protein expression levels of HLA-DPA1, HLA-DPB1 and CIITA were significantly decreased in the IL-13-treated group compared with the control group. (C) PBMCs were collected from children with asthma and healthy controls. Quantitative qPCR analysis revealed that the mRNA expression levels of HLA-DPA1 and HLA-DPB1 were significantly lower in PBMCs from the asthma group compared with the healthy control group. (D) Western blot analysis was performed to assess the protein expression levels of HLA-DPA1 and HLA-DPB1 in PBMCs. The results demonstrated that the protein levels of both genes were markedly downregulated in the asthma group compared with the healthy control group. GAPDH was used as a loading control. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001, Student’s t-test, one-way ANOVA, post hoc comparisons, Tukey’s test.

    Journal: Frontiers in Immunology

    Article Title: Integrative multi-omics reveals that downregulation of HLA-DPA1/DPB1 drives macrophage immune-metabolic dysregulation in pediatric asthma

    doi: 10.3389/fimmu.2026.1835475

    Figure Lengend Snippet: Experimental validation of HLA-DPA1 and HLA-DPB1 downregulation. (A) BEAS-2B human bronchial epithelial cells were treated with IL-13 (20 ng/mL) for 24 h to establish an in vitro asthma cell model. Quantitative real-time PCR analysis showed that the mRNA expression levels of HLA-DPA1, HLA-DPB1 and CIITA were significantly decreased in the IL-13-treated group compared with the control group. (B) BEAS-2B human bronchial epithelial cells were treated with IL-13 (20 ng/mL) for 24 h to establish an in vitro asthma cell model. Western blot analysis showed that the protein expression levels of HLA-DPA1, HLA-DPB1 and CIITA were significantly decreased in the IL-13-treated group compared with the control group. (C) PBMCs were collected from children with asthma and healthy controls. Quantitative qPCR analysis revealed that the mRNA expression levels of HLA-DPA1 and HLA-DPB1 were significantly lower in PBMCs from the asthma group compared with the healthy control group. (D) Western blot analysis was performed to assess the protein expression levels of HLA-DPA1 and HLA-DPB1 in PBMCs. The results demonstrated that the protein levels of both genes were markedly downregulated in the asthma group compared with the healthy control group. GAPDH was used as a loading control. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001, Student’s t-test, one-way ANOVA, post hoc comparisons, Tukey’s test.

    Article Snippet: BEAS-2B (normal human bronchial epithelial) cells were purchased from ATCC and maintained in DMEM at 37 °C.

    Techniques: Biomarker Discovery, In Vitro, Real-time Polymerase Chain Reaction, Expressing, Control, Western Blot